This study aimed to evaluate the influence of environmental conditions on milk yield and selected physicochemical properties of Iraqi buffalo milk in Babylon and Basrah Governorates during the period from December 2025 to February 2026. A total of 40 lactating buffaloes were included in the study and managed under a uniform feeding and husbandry system. Milk samples were analyzed for freezing point, relative density, and solids-not-fat (SNF) using a Lacto Flash–Funke Gerber analyzer.The results showed a progressive decline in both daily and monthly milk yield in the two governorates, accompanied by an increase in SNF and relative density, as well as a decrease in freezing point over the study period. Buffaloes in Babylon Governorate recorded higher milk production compared with those in Basrah Governorate, which may be attributed to more moderate environmental conditions in Babylon. The findings indicate that climatic and environmental factors play a significant role in determining milk productivity and composition traits in Iraqi buffaloes.