As one of the useful biological indicators for assessing water quality, aquatic beetles (Coleoptera) with two families, Dytiscidae and Hydrophilidae, have the highest species diversity and diversity in the world. This study is an attempt to identify the aquatic beetles of the Semirom region, Isfahan, Iran. Sampling was done from eight stations in Semirom area for year with the help of Sachuk. Samples taken in the laboratory were separated using tweezers and transferred to bottles containing 70% alcohol. The samples were first identified at the species level using valid identification keys and relevant sources and compared with samples recently collected and identified. The results showed that the collected samples belonged to five families, nine genera and ten species (Agabus bipustulatus, Hydroglyphus geminus, Agabus sturmii, Peltodytes caesus, Noterus clavicornis, Ilybius fuliginosus, Laccobius bipunctatus, Dryops ernesti, Enochrus tentaceus, Coelostoma orbiculare). Two species, named Agabus bipustulatus and Hydroglyphus geminus, were the most commonly preserved specimens.